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The valine metabolite 3-HIB promotes trans-endothelial fatty acid transport and ectopic lipid accumulation within skeletal muscle, contributing to local lipotoxicity and insulin resistance (23, 91)
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The cells were washed with PBS and incubated with FITC-tagged exendin 9–39 dissolved in cold PBS at a concentration of 10 μM for 30 min at 4 °C
